| Species | Name Used in Reference |
|---|---|
| Gambusia gaigei Hubbs, 1929 | Gambusia gaigei (p. ) |
| Gambusia hurtadoi Hubbs & Springer, 1957 | Gambusia hurtadoi (p. ) |
| Gambusia nobilis (Baird & Girard, 1853) | Gambusia nobilis (p. ) |
| Gambusia regani Hubbs, 1926 | Gambusia regani (p. ) |
| Gambusia vittata Hubbs, 1926 | Gambusia vittata (p. ) |