| Scientific Name | Author | English Name |
|---|---|---|
| Cualac tessellatus | Miller, 1956 | Checkered pupfish |
| Cubanichthys cubensis | (Eigenmann, 1903) | Cuban killifish |
| Cubanichthys pengelleyi | (Fowler, 1939) | Jamaican killifish |
| Cyprinodon alvarezi | Miller, 1976 | Potosi pupfish |
| Cyprinodon arcuatus | Minckley & Miller, 2002 | Santa Cruz pupfish |
| Cyprinodon artifrons | Hubbs, 1936 | Yucatan pupfish |
| Cyprinodon atrorus | Miller, 1968 | Bolson pupfish |
| Cyprinodon beltrani | Álvarez, 1949 | Blackfin pupfish |
| Cyprinodon bifasciatus | Miller, 1968 | Cuatro Cienegas pupfish |
| Cyprinodon bobmilleri | Lozano-Vilano & Contreras-Balderas, 1999 | San Ignacio pupfish |
| Cyprinodon bondi | Myers, 1935 | Hispaniola pupfish |
| Cyprinodon bovinus | Baird & Girard, 1853 | Leon Springs pupfish |
| Cyprinodon brontotheroides | Martin & Wainwright, 2013 | |
| Cyprinodon ceciliae | Lozano-Vilano & Contreras-Balderas, 1993 | Villa Lopez pupfish |
| Cyprinodon dearborni | Meek, 1909 | |
| Cyprinodon desquamator | Martin & Wainwright, 2013 | |
| Cyprinodon diabolis | Wales, 1930 | Devils Hole pupfish |
| Cyprinodon albivelis | Minckley & Miller, 2002 | Whitefin pupfish |
| Cyprinodon elegans | Baird & Girard, 1853 | Comanche Springs pupfish |
| Cyprinodon eremus | Miller & Fuiman, 1987 | Sonoyta pupfish |
| Cyprinodon esconditus | Strecker, 2002 | Hidden pupfish |
| Cyprinodon eximius | Girard, 1859 | Conchos pupfish |
| Cyprinodon fontinalis | Smith & Miller, 1980 | Carbonera pupfish |
| Cyprinodon higuey | Rodriguez & Smith, 1990 | |
| Cyprinodon hubbsi | Carr, 1936 | Lake Eustis minnow |
| Cyprinodon inmemoriam | Lozano-Vilano & Contreras-Balderas, 1993 | Charco Azul pupfish |
| Cyprinodon julimes | De la Maza-Benignos & Vela-Valladares, 2009 | |
| Cyprinodon labiosus | Humphries & Miller, 1981 | Thicklip pupfish |
| Cyprinodon laciniatus | Hubbs & Miller, 1942 | Bahama pupfish |
| Cyprinodon latifasciatus | Garman, 1881 | Parras pupfish |
| Cyprinodon longidorsalis | Lozano-Vilano & Contreras-Balderas, 1993 | La Palma pupfish |
| Cyprinodon macrolepis | Miller, 1976 | Largescale pupfish |
| Cyprinodon macularius | Baird & Girard, 1853 | Desert pupfish |
| Cyprinodon maya | Humphries & Miller, 1981 | Maya pupfish |
| Cyprinodon meeki | Miller, 1976 | Mezquital pupfish |
| Cyprinodon nazas | Miller, 1976 | Nazas pupfish |
| Cyprinodon nevadensis | Eigenmann & Eigenmann, 1889 | Amargosa pupfish |
| Cyprinodon nichollsi | Smith, 1989 | |
| Cyprinodon pachycephalus | Minckley & Minckley, 1986 | Bighead pupfish |
| Cyprinodon pecosensis | Echelle & Echelle, 1978 | Pecos pupfish |
| Cyprinodon pisteri | Miller & Minckley, 2002 | Palomas pupfish |
| Cyprinodon radiosus | Miller, 1948 | Owens pupfish |
| Cyprinodon riverendi | (Poey, 1860) | |
| Cyprinodon rubrofluviatilis | Fowler, 1916 | Red River pupfish |
| Cyprinodon salinus | Miller, 1943 | Salt Creek pupfish |
| Cyprinodon salvadori | Lozano-Vilano, 2002 | Bocochi pupfish |
| Cyprinodon simus | Humphries & Miller, 1981 | Boxer pupfish |
| Cyprinodon suavium | Strecker, 2005 | |
| Cyprinodon tularosa | Miller & Echelle, 1975 | White Sands pupfish |
| Cyprinodon variegatus | Lacepède, 1803 | Sheepshead minnow |
| Cyprinodon verecundus | Humphries, 1984 | Largefin pupfish |
| Cyprinodon veronicae | Lozano-Vilano & Contreras-Balderas, 1993 | Charco Palma pupfish |
| Floridichthys carpio | (Günther, 1866) | Goldspotted killifish |
| Floridichthys polyommus | Hubbs, 1936 | Ocellated killifish |
| Garmanella pulchra | Hubbs, 1936 | Yucatan flagfish |
| Jordanella floridae | Goode & Bean, 1879 | Flagfish |
| Megupsilon aporus | Miller & Walters, 1972 | Catarina pupfish |
| Orestias agassizii | Valenciennes, 1846 | |
| Orestias albus | Valenciennes, 1846 | |
| Orestias ascotanensis | Parenti, 1984 | |
| Orestias chungarensis | Vila & Pinto, 1987 | |
| Orestias crawfordi | Tchernavin, 1944 | |
| Orestias ctenolepis | Parenti, 1984 | |
| Orestias cuvieri | Valenciennes, 1846 | Orestias |
| Orestias elegans | Garman, 1895 | |
| Orestias empyraeus | Allen, 1942 | |
| Orestias forgeti | Lauzanne, 1981 | |
| Orestias frontosus | Cope, 1876 | |
| Orestias gilsoni | Tchernavin, 1944 | |
| Orestias gloriae | Vila, Scott, Mendez, Valenzuela, Iturra & Poulin, 2012 | |
| Orestias gracilis | Parenti, 1984 | |
| Orestias gymnota | Parenti, 1984 | |
| Orestias hardini | Parenti, 1984 | |
| Orestias imarpe | Parenti, 1984 | |
| Orestias incae | Garman, 1895 | |
| Orestias ispi | Lauzanne, 1981 | |
| Orestias jussiei | Valenciennes, 1846 | |
| Orestias lastarriae | Philippi, 1876 | |
| Orestias laucaensis | Arratia, 1982 | |
| Orestias luteus | Valenciennes, 1846 | |
| Orestias minimus | Tchernavin, 1944 | |
| Orestias minutus | Tchernavin, 1944 | |
| Orestias mooni | Tchernavin, 1944 | |
| Orestias mulleri | Valenciennes, 1846 | |
| Orestias multiporis | Parenti, 1984 | |
| Orestias mundus | Parenti, 1984 | |
| Orestias olivaceus | Garman, 1895 | |
| Orestias parinacotensis | Arratia, 1982 | |
| Orestias pentlandii | Valenciennes, 1846 | |
| Orestias piacotensis | Vila, 2006 | |
| Orestias polonorum | Tchernavin, 1944 | |
| Orestias puni | Tchernavin, 1944 | |
| Orestias richersoni | Parenti, 1984 | |
| Orestias robustus | Parenti, 1984 | |
| Orestias silustani | Allen, 1942 | |
| Orestias taquiri | Tchernavin, 1944 | |
| Orestias tchernavini | Lauzanne, 1981 | |
| Orestias tomcooni | Parenti, 1984 | |
| Orestias tschudii | Castelnau, 1855 | |
| Orestias tutini | Tchernavin, 1944 | |
| Orestias uruni | Tchernavin, 1944 | |
| Orestias ututo | Parenti, 1984 | |
| Pseudorestias lirimensis | Arratia, Vila, Lam, Guerrero & Quezada-Romegialli, 2017 |